The Life of Osteoclasts
Year
March, 2002
Time
20 min.
Summary
Bone is continually remodeled by osteoblasts which deposit fresh layers of bone and osteoclasts which erode bone matrix. Previous studies demonstrated that osteoclasts are differentiated from monocytes/macrophages in bone marrow.
The aims of this film are to record the site where osteoclasts are born and how they grow and acquire functions. We could film in microscopic image that cells collected from bone marrow matured and differentiated to osteoclasts.
Furthermore, the effect of calcitonin, one of bone metabolism-related hormones, was observed under microscope controlling differentiation and function of osteoclasts. Actin and tubulin fibers were visualized by fluorescent staining, and we could observe for the first time the dynamic change of these cytoskeletons in the osteoclasts.
It can be said that this is the first video to demonstrate the life of osteoclasts with the microscope image.
The aims of this film are to record the site where osteoclasts are born and how they grow and acquire functions. We could film in microscopic image that cells collected from bone marrow matured and differentiated to osteoclasts.
Furthermore, the effect of calcitonin, one of bone metabolism-related hormones, was observed under microscope controlling differentiation and function of osteoclasts. Actin and tubulin fibers were visualized by fluorescent staining, and we could observe for the first time the dynamic change of these cytoskeletons in the osteoclasts.
It can be said that this is the first video to demonstrate the life of osteoclasts with the microscope image.
Awards
2003 科学技術映像祭基礎研究部門文部科学大臣賞
2003 TEPIAハイテク・ビデオ・コンクール奨励賞
2003 日本産業映画・ビデオコンクール学術・研究部門奨励賞
2003 TEPIAハイテク・ビデオ・コンクール奨励賞
2003 日本産業映画・ビデオコンクール学術・研究部門奨励賞
Planning
Asahi Kasei Corp.
※The name of a planning company, supervisor and their title is that at the time of completion.
Staff
Director : Ozawa, Eiji
Screenplay : Ozawa, Eiji
Cinematographer :Hayashi, Masahiro / (SEM)Kitahara, Yukio
Experiments : Tomita, Tsutomu / Ma, Jiun / (SEM)Ishizaki, Michiko
CGI : Nagata, Masami
Narrator : Ito, Souichi
Production Manager : Todoroki, Yasuyuki
Producer : Yoshino, Toshimasa
Screenplay : Ozawa, Eiji
Cinematographer :Hayashi, Masahiro / (SEM)Kitahara, Yukio
Experiments : Tomita, Tsutomu / Ma, Jiun / (SEM)Ishizaki, Michiko
CGI : Nagata, Masami
Narrator : Ito, Souichi
Production Manager : Todoroki, Yasuyuki
Producer : Yoshino, Toshimasa